What is BrdU proliferation assay?
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What is BrdU proliferation assay?
General description. BrdU Cell Proliferation Assay is a non-isotopic immunoassay for quantification of BrdU incorporation into newly synthesized DNA of actively proliferating cells. It is sensitive, rapid, and easy to perform.
What is BrdU incorporation assay?
Bromodeoxyuridine (BrdU) incorporation assays have long been used to detect DNA synthesis in vivo and in vitro. The key principle of this method is that BrdU incorporated as a thymidine analog into nuclear DNA represents a label that can be tracked using antibody probes.
What is proliferation assay?
Cell proliferation assays are designed to quantify the relative rates of cell division within such target tissues using specialized immunohistochemical staining techniques to detect proliferating cells.
Why do we do proliferation assay?
Cell proliferation assays typically detect changes in the number of cells in a division or changes in a cell population.
What kind of cells does BrdU identify?
BrdU (Bromodeoxyuridine / 5-bromo-2′-deoxyuridine) is an analog of the nucleoside thymidine used in the BrdU assay to identify proliferating cells. BrdU labeling can be performed in vitro for cell lines and primary cell cultures, or in vivo for labeling cells within a living animal.
How do you measure proliferation?
Measuring by metabolic activity A tried-and-true method of measuring proliferation by metabolism is using tetrazolium salts such as MTT, MTS or XTT. The salts are reduced by metabolically active cells to a colored formazan, which is then detected using a spectrophotometer.
What is growth assay?
This method allows one to distinguish live from dead cells and the increase in number of cells provides a measure of cell proliferation. Furthermore, the procedure is suitable for high-throughput experiments. Some proliferation assays measure the increase in metabolic activity due to an increase in cell number.
