How does a FLAG-tag work?
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How does a FLAG-tag work?
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence.
What are protein tags used for?
Protein tags are a useful and convenient tool for improving solubility of recombinant proteins, streamlining protein purification, and allowing an easy way to track proteins during protein expression and purification.
Why tags are used in protein purification?
Protein tagging provides a powerful alternative to antibodies and can be used in living systems. It involves cloning a gene of interest in-frame with a protein tag that will be expressed as a fusion with the resulting protein—and that allows the protein to be captured or detected.
What does flag mean in biology?
FLAG tag (DYKDDDDK) is an epitope tag widely adopted for its high specificity and purity in protein purification. Anecdotally, it is commonly used as a follow-on or alternative to His tag when high purity is desired.
What is flag in immunoprecipitation?
Flag®-tag (or DYKDDDDK-tag) is a commonly used short peptide tag for multiple applications such as immunoprecipitation (IP), protein purification, immunofluorescence, and Western blotting (WB).
What is a flag HA tag?
The FLAG® HA tandem epitope system consists of small epitopes tags that are not eukaryotic derived. These features minimize interference with protein functions and provide superior specificity.
Where do you tag proteins?
They can be added to either end of the target protein, so they are either C-terminus or N-terminus specific or are both C-terminus and N-terminus specific. Some tags are also inserted into the coding sequence of the protein of interest; they are known as internal tags.
What is flag immunoprecipitation?
Immunoprecipitation (IP) can be used for efficient, high-yield isolation and purification of proteins fused to the FLAG® peptide tag. IP is performed with the ANTI-FLAG® M2 affinity gel, which is a highly specific monoclonal antibody covalently bound to agarose resin.
What is flag in co immunoprecipitation?
What is Flag immunoprecipitation?
