What does Giemsa stain test for?
Table of Contents
What does Giemsa stain test for?
Giemsa stain is a gold standard staining technique that is used for both thin and thick smears to examine blood for malaria parasites, a routine check-up for other blood parasites and to morphologically differentiate the nuclear and cytoplasm of Erythrocytes, leucocytes and Platelets and parasites.
What is Giemsa stain and how is it used?
Giemsa stain is mainly used for staining of peripheral blood smears and specimens obtained from the bone marrow. It is used to obtain differential white blood cell counts. Giemsa stain is also used in cytogenetics to stain the chromosomes and identify chromosomal aberrations.
Which stain is used for staining blood film?
Commonly used stain in our environment is Leishman stain which is composed of polychrome methylene blue (basic component) and eosin (acidic component). May-Grunwald Giemsa or Wright-Giemsa stain can also be used.
What does Giemsa stain bind to?
Giemsa is a visible light dye that binds to DNA through intercalation and thus, is used for chromosome staining. It is a mixture of cationic thiazine dyes, most importantly azure B, and anionic eosin dyes such as eosin Y (figure 4.1) [16].
What Colour is Giemsa stain?
Giemsa stain is one of the best known histological stains, coloring the nuclei dark blue and the cytoplasm blue to pink, according to the acidity of the cytoplasmic contents.
What is Giemsa stain used for in histology?
The Giemsa staining method was adapted for histology because of its uses demonstrating chromatin and the nuclear membrane. It is used for the detection of Helicobacter pylori bacteria, which grows in the digestive tract and can cause cancer.
Which structure does Giemsa stain?
Giemsa staining giemsa It stains red blood cells in red/pink and platelets in light pink. The leukocyte nuclear chromatin appears in magenta (Figure 1). Giemsa specifically binds to phosphate groups of DNA, in particular to regions of the DNA that is rich in adenine-thymine bonding.
What structures does Giemsa stain?
What are the types of Giemsa banding?
By applying specific stains, the banding patterns become apparent. Other types of banding are reverse (R-) banding, constitutive heterochromatin (C-) banding, quinacrine (Q-) banding, Nucleolar Organizer Region (NOR-) banding, and telomeric R (T-) banding.
Which structures does Giemsa stain?
How Giemsa stain is prepared?
Stock Solution:
- Dissolve 1g of Giemsa (MERCK) powder into 54 ml of glycerin.
- Heat this solution at ~60oC about 1.30h- 2h. Let cool to room temperature.
- Add 84 ml of methanol.
- Let the bottle tightly closed and protected from light for one week.
- Filter the solution and keep protected from light.
Which banding is used for Giemsa dye?
C-banding is specifically used for identifying heterochromatin by denaturing chromosomes in a saturated alkaline solution followed by Giemsa staining.
What is the difference between G-banding and C-banding?
G-banding involves the staining of trypsin-treated chromosomes and R-banding involves denaturing in hot acidic saline followed by Giemsa staining. C-banding is specifically used for identifying heterochromatin by denaturing chromosomes in a saturated alkaline solution followed by Giemsa staining.
