What is the molecular weight of GAPDH?
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What is the molecular weight of GAPDH?
36 kDa
GAPDH molecule is a homotetramer composed of 36 kDa subunits. Thus the molecular weight for the whole molecule is 144 kDa.
How many kDa is GAPDH?
GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. There is no cross-reaction with GAPDH from yeast. Preliminary data indicates that the GAPDH antibody- loading control ab8245 recognizes the monomer (36 kDa) and also the dimer forms of GAPDH, but not the tetrameric form of the protein.
What does GAPDH do in Western blot?
GAPDH antibody, is usually used as a loading control antibody for Western Blot to normalize the levels of protein detected by confirming that protein loading is the same across the gel.
What is anti GAPDH?
Antibodies that detect GAPDH can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunohistochemistry and ELISA. These antibodies target GAPDH in Human, Mouse, Rat, Rabbit and Yeast samples.
How many base pairs is GAPDH?
(41) for the human GAPDH gene expressed in HeLa cells. An active promoter was located within 112 base pairs 5′ to the start site of transcription of this gene.
Why is GAPDH used in PCR?
As one of the most common reference genes, GAPDH is often used to normalize the gene expression data, being used as an endogenous control in the quantitative analysis of RT-PCR, since in some experimental systems, its expression is very constant (Edwards and Denhardt 1985; Winer et al. 1999).
What is GAPDH primer?
Primers specific to the gene GAPDH, or glyceraldehyde 3-phosphate dehydrogenase, are often used in PCR-based experiments as a type of control. GAPDH is an enzyme involved in glycolysis and is widely expressed among many mammalian cell types.
What is the function of GAPDH?
Abstract. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has long been recognized as an important enzyme for energy metabolism and the production of ATP and pyruvate through anaerobic glycolysis in the cytoplasm.
Where is GAPDH?
In addition to the cytoplasm where majority of GAPDH is located under the basal condition, GAPDH is also found in the particulate fractions, such as the nucleus, the mitochondria, and the small vesicular fractions. When cells are exposed to various stressors, dynamic subcellular re-distribution of GAPDH occurs.
How big is the GAPDH protein?
37-kDa
Under normal cellular conditions, cytoplasmic GAPDH exists primarily as a tetramer. This form is composed of four identical 37-kDa subunits containing a single catalytic thiol group each and critical to the enzyme’s catalytic function.
What type of gene is GAPDH?
Protein Coding gene
GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) is a Protein Coding gene.
Why GAPDH is a housekeeping gene?
GAPDH is a key regulatory enzyme, which catalyzes the oxidative phosphorylation of glyceraldehyde-3-phosphate during glycolysis. β-actin is a cytoskeletal protein involved in cell structure and motility. Based on these basic and ubiquitous cellular functions, GAPDH and β-actin are considered as housekeeping genes.
Why is GAPDH a housekeeping gene?
What does GAPDH do in glycolysis?
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme involved in breaking down glucose to obtain energy. More specifically, in eukaryotes, this enzyme catalyzes the sixth step in glycolysis, converting glyceraldehyde 3-phosphate to D-glycerate 1,3-bisphosphate (1,3-BPG).
Does GAPDH use NADH?
GAPDH catalyzes the transformation of glyceraldehyde 3-phosphate to glycerate-1, 3-biphosphate, a process accompanied by the production of NADH.
What class of enzyme is GAPDH?
glycolytic enzyme
GapDH is a key glycolytic enzyme, utilizing NAD+ as a coenzyme for the oxidative phosphorylation of glyceraldehyde-3-phosphate to 1,3-diphosphoglycerate.
What kind of enzyme is GAPDH?
Glyceraldehyde 3-phosphate dehydrogenase (abbreviated GAPDH) (EC 1.2. 1.12) is an enzyme of about 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules.
