What is the detection LImit of NanoDrop?
Table of Contents
What is the detection LImit of NanoDrop?
NanoDrop
| Sample | Lower Detection Limit | Upper Detection LImit |
|---|---|---|
| Nucleic acids | 2.5 ng/µl | 3,700 ng/µl (dsDNA) 3,000 ng/µl (RNA) 2,400 ng/µl (ssDNA) |
| Purified BSA | 0.15 mg/ml | 100 mg/ml |
| Protein Pierce Assay | 50 µg/ml | 2,000 µg/ml |
| MicroArray Fluorescent Dyes (Cy3, Cy5, Alexa Fluors, etc.) | 0.17 -0.50 pmol/µl | 60 – 215 pmol/µl |
What is a good concentration for NanoDrop?
Look out below! The NanoDrop’s Linear Dynamic Range
| Model | Linear Range (ng/µL) | Linear Range (mg/mL) |
|---|---|---|
| NanoDrop 2000 | 2–15,000 | 0.1–400 |
| NanoDrop 8000 | 2.5–3700 | 0.1–100 |
| NanoDrop One | 0.2–27,500 | 0.06–820 |
| NanoDrop Eight | 0.2–10,000 | 0.06–300 |
Is NanoDrop or Qubit more accurate?
The sensitivity of the Qubit can be as low as 10 pg/mL, which is far superior to the Nanodrop. So, if you worry your samples have very low concentrations, it is best to use the Qubit. Another advantage the Qubit has over the Nanodrop is its specificity.
How do you interpret NanoDrop results?
There should be a nice peak at 260 nm, which indicates the presence of nucleic acids, and no peaks elsewhere. If there are double peaks or shifts in the curve, these are vital warning signs that the sample is not pure.
What is a good NanoDrop reading?
What does a low A260 A230 mean?
260/230 Ratios Abnormal 260/230 values may indicate a problem with the sample or with the extraction procedure, so it is important to consider both. A low A260/A230 ratio may be the result of: • Carbohydrate carryover (often a problem with plants). • Residual phenol from nucleic acid extraction.
What does a low 260 230 ratio indicate?
260/230 Ratios Abnormal 260/230 values may indicate a problem with the sample or with the extraction procedure, so it is important to consider both. A low A260/A230 ratio may be the result of: • Carbohydrate carryover (often a problem with plants). Residual phenol from nucleic acid extraction.
What does a low A260 A280 ratio mean?
Abnormal 260/280 ratios usually indicate that the sample is either contaminated by protein or a reagent such as phenol or that there was an issue with the measurement. A low A260/A280 ratio may be caused by: • Residual phenol or other reagent associated with the. extraction protocol.
What is a good 260 230 ratio?
Generally acceptable 260/230 ratios are in the range of 2.0 – 2.2. Values higher than this may indicate contamination with the aforementioned compounds.
What does a low A260 a280 ratio mean?
Common Problems. Abnormal 260/280 ratios usually indicate that a sample is contaminated by residual phenol, guanidine, or other reagent used in the extraction protocol, in which case the ratio is normally low. Inaccurate ratios may also be encountered at very low concentrations (< 10 ng/µl) of nucleic acids.
