How do you test the amount of vitamin C in orange juice?

How do you test the amount of vitamin C in orange juice?

One way to determine the amount of vitamin C in juice is to use a redox titration. In a redox titration, one compound donates electrons to another compound. The compound losing electrons is oxidized and the compound gaining electrons is reduced.

What affects the concentration of vitamin C in orange juice?

Given its unstable nature and oxidative property, many factors may have the potential of affecting the vitamin C concentration of fresh orange juice. These factors include temperature, type of metal storage container, and sugar additive.

What is the role of orange juice in the experiment?

In the hypothesis it stated that the home squeezed orange juice would have the most Vitamin C, which was proved true with the experiment. In this case, the experiment showed that the orange juice from concentrate had the least amount, because it changed color the quickest.

Does the level of vitamin C in orange juice change over time experiment?

It was evident through our data that the ascorbic acid content changed dramatically over time. The orange juice became more intense over the content of 6 weeks, with each sample ascorbic levels increasing or staying the same. This is evident in the change in % ascorbic acid content over time.

How do you test vitamin C in juice?

Divide the total number of iodine drops by the number of drops per mg of vitamin C from the reference sample. For example, if your test fruit required 10 drops of iodine, and the reference sample required 2 drops per 1 mg of vitamin C–the formula would be 10/2=5 or 5 mg of vitamin C per ounce of fruit juice.

How do you test for vitamin C in science?

Take a small (known) amount – 50 mL for example – of the vitamin C solution and add five drops of starch indicator. To your mixture of vitamin C and starch indicator, add drops of iodine, carefully counting the drops, until the solution develops a blue-black colour that does not disappear on stirring.

How does temperature affect vitamin C in orange juice?

As the temperature increases, the concentration of vitamin C will decrease. As a result, smaller amount of potassium iodate will be involved.

How do you test for vitamin C in fruit?

Which type of orange juice has the most vitamin C experiment?

The fresh-squeezed orange juice had 43% more Vitamin C (ascorbic acid) than the premium orange juice and 40% more Vitamin C than the orange juice made from frozen concentrate. It took 65 drops of iodine solution to make the fresh-squeezed orange juice change color (run out of Vitamin C).

How do you test for vitamin C?

Test it by adding a few drops of indicator to a few drops of tincture of iodine dissolved in water: if it has been prepared properly the indicator solution should develop a blue-black colour upon addition of the iodine.

What colour is a positive vitamin C test?

What’s Going On? The indicator solution turns from blue to clear when you add a drop or two of vitamin C. This positive test shows you how the indicator solution works: if the blue disappears, your liquid contains vitamin C. This procedure is called a titration.

How do you test for vitamin C in juice?

How do you test vitamin C for fruit juice?

Why does vitamin C decrease with temperature?

High-heat cooking temperatures or prolonged cook times can break down the vitamin. Because it is water-soluble, the vitamin can also seep into cooking liquid and be lost if the liquids are not eaten.

Does temperature affect vitamin C content?

Vitamin C is a water-soluble and temperature-sensitive vitamin, so is easily degraded during cooking, and elevated temperatures and long cooking times have been found to cause particularly severe losses of vitamin C [12].

How do you test for vitamin C in chemistry?

teaspoon of Tang® powder to 2 tablespoons of water in your fifth cup. Label this cup Tang Drink. 7 Try adding 1 drop of Tang® drink to the test cup. If it takes more than 1 drop to clear the solution, that means there is less vitamin C in 1 drop of Tang than there is in 1 drop of your vitamin C solution.

What is a common test for the presence of vitamin C?

Tincture of iodine is brownish-red in color. When vitamin C is added, it becomes colorless. This reaction can be used to test for the presence of vitamin C and to give some idea of the amount that can be found in a given substance.

What does the positive test for vitamin C look like?

Which orange juice has the most vitamin C experiment?

The fresh-squeezed orange juice had 43% more Vitamin C (ascorbic acid) than the premium orange juice and 40% more Vitamin C than the orange juice made from frozen concentrate.