What are Transwell inserts used for?
Table of Contents
What are Transwell inserts used for?
Transwell inserts provide an updated technique for culturing polarized cells because the membrane permits the cells to uptake and secrete molecules on both their basal and apical surfaces and fosters metabolic activities that better mimic those that occur in the body.
What is transwell system?
Transwell® Permeable Supports are convenient and easy-to-use devices for studies of both anchorage-dependent and anchorage- independent cells. These inserts provide independent access to both sides of a monolayer, thus giving researchers a versatile tool to study transport and other metabolic activities in vitro.
Can you reuse Transwell inserts?
In summary, Transwell inserts can be reused after cell removal and membrane washing when inserts are maintained in sterile conditions. Multiple measurements/assays suggest that no significant negative impact is incurred by insert reuse.
How do you cut a transwell membrane?
To remove the membrane from the insert, use forcepts and pick up the Transwell insert and flip upside down. Cut the membrane off by using a sharp scalpel. Slowly cut around the inner circum- ference of the membrane. If the membrane starts to curl, adjust the angle or speed of the cutting to reduce the curling effect.
How many cells are in a Transwell?
Cells (5 × 104 -2 x105 cells per well) were seeded in top chambers of the transwell plates in FBS-free media with membrane inserts without matrigel coated.
What is a Transwell plate?
Corning™ Transwell™ Multiple Well Plate with Permeable Polycarbonate Membrane Inserts. Patented self-centering design provides independent access to both apical and basolateral membranes of the cell monolayer.
What is Transwell cell culture?
Transwell cell culture inserts are convenient, easy-to-use permeable support devices for the study of both anchorage-dependent and anchorage-independent cell lines. They feature a 10 μm thin transparent polycarbonate membrane available in a variety of pore sizes. Transwell are treated for optimal cell attachment.
How many cells in a 24-well plate well?
Useful information for various sizes of cell culture dishes and flasks
| Catalog No. | Cells at confluency* | |
|---|---|---|
| Dishes | ||
| 24-well | 142475 | 0.24 x 106 |
| 48-well | 150687 | 0.12 x 106 |
| 96-well | 167008 | 0.04 x 106 |
How much volume does a 24-well plate have?
0.5-2.39 mL
CulturPlate Microplates
| Well format | 24-well | 96-well |
|---|---|---|
| Recommended working volume | 0.5-2.39 mL | 80 µL-350 µL |
| Height (mm) | 18.70 | 14.60 |
| Length (mm) | 127.80 | 127.76 |
| Width (mm) | 85.60 | 85.47 |
What is a 24 well plate used for?
AcroPrep 24-well filter plates with Omega membrane can be used for ultrafiltration applications including desalting, diafiltration, concentration or fractionation of protein and nucleic acid samples.
How many cells do you need for a 24 well plate?
What is a 24 well plate made of?
Although microplates are made from a variety of materials, the most common material is polystyrene.
How much volume does a 24 well plate have?
CulturPlate Microplates
| Well format | 24-well | 384-well |
|---|---|---|
| Recommended working volume | 0.5-2.39 mL | 24 µL-90 µL |
| Height (mm) | 18.70 | 14.35 |
| Length (mm) | 127.80 | 127.76 |
| Width (mm) | 85.60 | 85.47 |
How many cells are in a 24 well plate?
Useful information for various sizes of cell culture dishes and flasks
| Catalog No. | Cells at confluency* | |
|---|---|---|
| Dishes | ||
| 6-well | 140675 | 1.2 x 106 |
| 12-well | 150628 | 0.5 x 106 |
| 24-well | 142475 | 0.24 x 106 |
What is the purpose of a well plate?
noun. A rectangular plate or block containing multiple small wells or cavities, used for analysis, clinical testing, or culture; specifically = microtiter plate .
