What is CytoLyt made of?
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What is CytoLyt made of?
Product Description: A methanol based, buffered preservative solution. Product Use: A preservative solution to support cells during transport and slide preparation. Manufacturer: Hologic Inc. EMERGENCY OVERVIEW: Warning!
What is CytoLyt solution used for?
CytoLyt Solution is a media used for collection and lysing of red blood cells. When used on samples processed with a ThinPrep processor, the process allows transfer of cells onto a microscope slide, providing a thin, uniform layer of cells suitable for cytologic evaluation.
How do you dispose of ThinPrep CytoLyt solution?
In case of fire: Use CO2, dry chemical, or foam for extinction. Dispose of contents/container to an approved waste disposal plant.
What is ThinPrep solution?
SOLUTIONS: The ThinPrep process uses PreservCyt and CytoLyt® solutions. The PreservCyt is a methanol- based, buffered preservative solution used to support cells during transport and slide preparation. The CytoLyt is a methanol-based, buffered transport solution used in specimen preparation prior to processing.
What is PreservCyt solution?
PreservCyt Solution is a methanol-based reagent that serves as a transport, preservative, and antibacterial medium for gynecologic samples. PreservCyt Solution is also a collection and transport medium for gynecologic samples for certain tests for Human Papilloma Virus (HPV) and other sexually transmitted infections.
What is used as fixative?
The most commonly used fixative in histology is formaldehyde. It is usually used as a 10% neutral buffered formalin (NBF), that is approx. 3.7%–4.0% formaldehyde in phosphate buffer, pH 7.
What is Thin Prep made of?
How long is a thin prep good for?
Store PreservCyt Solution with cytologic sample intended for ThinPrep Pap testing between 15°C (59°F) and 30°C (86°F) for up to 6 weeks. Line on cap and line on vial should meet or slightly overlap.
Is ethanol a fixative?
Methanol is commonly used as a fixative for blood films and 95% ethanol is used as a fixative for cytology smears but both alcohols are usually combined with other reagents when used as fixatives for tissue specimens.
How does ethanol fix cells?
Ethanol will cause distortion of nuclear and cytoplasmic detail, it will remove lipids while dehydrating the cells and also precipitate proteins on the cellular architecture. On the other hand, fixation can be done using crosslinking reagents, such as paraformaldehyde (as suggested above).
What is tested in Thin Prep?
The ThinPrep Pap test helps healthcare providers detect the presence of abnormal cervical cells, and the Aptima HPV assays identify high-risk HPV mRNA that is indicative of the HPV infections most likely to lead to cervical disease.
How do you make a ThinPrep?
Insert the brush into the cervix until only the bottom- most fibers are exposed. Slowly rotate 1/4 or 1/2 turn in one direction. DO NOT OVER-ROTATE. …the brush as quickly as possible in the PreservCyt Solution by rotating the device in the solution 10 times while pushing against the PreservCyt vial wall.
Can ThinPrep be refrigerated?
PLEASE NOTE: The specimen does not need to be refrigerated or packed with ice.
Why is alcohol used as a fixative?
Ethanol is a non-additive precipitant fixative. It fixes proteins by dehydration and precipitation, the degree to which this is done being dependant on the amount of water present and the solubility of the materials in the mixture.
Does ethanol damage the cell membrane?
Erythrocyte membranes isolated from chronic alcoholics exhibited an increase in cholesterol content and a decrease in membrane fluidity (Parmahamsa et al. 2004). These early studies clearly demonstrate that ethanol treatment leads to the physical disruption of lipid membranes in the cells.
How does ethanol destroy cell membrane?
As the ethanol concentration increases, membrane permeability will increase. If the ethanol concentration is high enough, enough phospholipids will dissolve to cause the plasma membrane to disintegrate completely which will kill the cell.
