What is diethylaminoethyl DEAE cellulose primarily used for?
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What is diethylaminoethyl DEAE cellulose primarily used for?
Diethylaminoethyl cellulose (DEAE-C) is a positively charged resin used in ion-exchange chromatography, a type of column chromatography, for the separation and purification of proteins and nucleic acids.
How does DEAE chromatography work?
DEAE-Sephadex is a positively-charged slurry that will have electrostatic interactions with the negatively charged atoms, making them elute later than the positively-charged molecules in the interested sample. This is a separation technique used widely to discover specific proteins, or enzymes in the body.
Is DEAE cellulose anion or cation exchanger?
At neutral pH the carboxymethyl group is ionized as -CH2OCH2COO¯ so that CM- cellulose is negatively charged, so it is a weak cation exchanger. DEAE-cellulose contains an diethylaminoethyl group. It is positively charged at neutral pH and so DEAE-cellulose is a weak anion exchanger.
Is DEAE a cation exchanger?
What is the nature of the ion exchanger in DEAE?
DEAE Sephacel™ is a weak anion exchanger based on beaded cellulose. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range, pH 2 to 9.
How can you separate proteins by their charges?
The other principal method of separating proteins by charge is to allow them to migrate along a pH gradient in an electric field, coming to rest at the pH at which their net charge is zero—the protein’s isoelectric point (Pi). This is the technology behind capillary isoelectric focusing (cIEF) instrumentation.
What are Sepharose beads?
Sepharose Big Beads are ion exchangers designed for large-scale industrial applications. The media are based on 100–300 μM, cross-linked 6% agarose particles, substituted with quaternary ammonium (Q) or sulfopropyl (SP) groups.
What is the composition of Sepharose?
Sepharose is a tradename for a crosslinked, beaded-form of agarose, a polysaccharide polymer material extracted from seaweed. Its brand name is a portmanteau derived from Separation-Pharmacia-Agarose. A common application for the material is in chromatographic separations of biomolecules.
Which technique is used to separate two identical proteins?
Chromatography: Chromatography is one of the most useful analytical techniques used in chemistry laboratories. It is the separation of the components on their compounds based on their polarity and size.
How do you separate proteins by size?
Size Exclusion Chromatography Size exclusion chromatography is a method of separating proteins and other molecules based on their size. Small molecules flow through the pore of the matrix and fit better than large molecules.
