What is the difference between C8 and C18 HPLC column?

What is the difference between C8 and C18 HPLC column?

C18 has 18 carbon atoms while C8 has only 8 carbon atoms. C18 has a longer carbon chain, but C8 has a shorter one. C18 has higher retention while C8 has shorter retention. C18 has higher hydrophobicity, but C8 has a lower hydrophobicity.

Which column is best for HPLC?

Types of HPLC Columns A few of the most commonly used types are Normal-Phase HPLC Columns, Reverse-Phase HPLC Columns, Ion-Exchange HPLC Columns, Size Exclusion HPLC Columns, Affinity HPLC Columns, etc[1,2]. These HPLC columns separate the analytes based on polarity, pore size, affinity and ion charges.

Why we use C8 column in HPLC?

C8 column is one of the columns used in HPLC in the reverse-phase. Generally, it contains octyl carbon chains, which are shorter. Therefore, its hydrophobicity is comparatively low. Also, this makes low retention time of nonpolar molecules in the column.

What is the difference between normal phase and reverse-phase?

In normal-phase chromatography, the stationary phase is polar and the mobile phase is nonpolar. In reversed phase we have just the opposite; the stationary phase is nonpolar and the mobile phase is polar.

What is difference between ODS and BDS column?

ODS and BDS are two columns used for reverse-phase chromatography. The key difference between ODS and BDS column is that ODS column contains free –OH functional groups, whereas BDS column contains deactivated –OH groups. Moreover, ODS columns have high peak tailing while BDS columns are designed to reduce peak tailing.

Why different columns are used in HPLC?

Different types of HPLC columns are used in analysis of different pharmaceutical compounds according to their nature and column separation capacity. Columns are the main component in HPLC because the column is responsible for the separation of the sample components.

Which HPLC is best?

Shimadzu makes the best HPLC pumps on the market I believe, also the UV and MS detectors are really, really good.

Is C8 column reverse phase?

C8 column is a type of column used in the reverse-phase chromatography. Generally, the reverse-phase chromatography uses a hydrophobic stationary phase. However, the solid support stationary phase or the column contains modified silica.

Is C8 column reverse-phase?

Is C8 polar or nonpolar?

Why is reversed phase HPLC preferred?

What are the Advantages of Reverse HPLC? By using water (or a water-based substance) as the solvent, reversed HPLC eliminates the danger of the analyte retention times being skewed due to absorption of water in the atmosphere.

What is C8 HPLC column?

What is C8 Column in HPLC. C8 column is a type of column used in the reverse-phase chromatography. Generally, the reverse-phase chromatography uses a hydrophobic stationary phase. However, the solid support stationary phase or the column contains modified silica. Here, silica is a hydrophilic stationary phase.

What is phenyl column?

Phenyl-Hexyl columns provide unique selectivity for aromatic compounds when compared to other reversed-phase packings such as C18 and C8. Unlike, the standard Phenyl column, Phenyl-Hexyl allows much greater retention and separation of the aromatic compounds through its extended hexyl hydrocarbon functional group.

What is C8 column?

C8 column is a type of column used in the reverse-phase chromatography. Generally, the reverse-phase chromatography uses a hydrophobic stationary phase. However, the solid support stationary phase or the column contains modified silica. Here, silica is a hydrophilic stationary phase.

What is the pH of HPLC water?

7
Specifications

Can I use distilled water for HPLC?

The quality of commercial HPLC grade distilled water is guaranteed for use in analysis using UV absorption detectors, as mentioned above, but it is not necessarily guaranteed for use in analyzing inorganic ions. Therefore, you should confirm that the water does not cause any analysis problems before using it.