What is the purpose of hektoen enteric?
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What is the purpose of hektoen enteric?
Hektoen Enteric Agar (HE) is a selective and differential medium designed to isolate and differentiate members of the species Salmonella and Shigella from other Enterobacteriaceae. Bile salts and the dyes bromthymol blue and acid fuchsin inihibit the growth of most Gram positive organisms.
What makes hektoen enteric agar selective?
Hektoen Enteric agar is a selective and differential medium for the isolation and differentiation of pathogens from clinical samples. ◈ Selectivity : The presence of bile salts and dyes inhibits most Gram-positive organisms, which only allows Gram-negative bacilli to develop on agar.
What are the characteristics of hektoen Agar?
Proteus strains are somewhat inhibited; colonies that develop are small transparent and more glistening or watery in appearance than species of Salmonella or Shigella….Result Interpretation on Hektoen Enteric Agar.
| Organisms | Growth |
|---|---|
| Salmonella Enteritidis | Blue-green with or without black centers |
Is Hektoen Enteric Agar defined or undefined?
HE agar is a complex (chemically undefined), moderately selective, and differential medium designed to isolate Salmonella and Shigella species from other enterics.
Is Hektoen Enteric Agar complex or chemically defined?
isolates and differentiates shigella and salmonella species from other gram negative enteric organisms based on their ability to: – overcome the inhibitory effects of bile. – reduce sulfur to H2S. – ferment lactose, sucrose, or salicin. is Hektoen-Enteric Agar complex or chemically defined.
Is Hektoen Enteric Agar a defined or an undefined medium provide the reasoning behind your choice and explain why this formulation is desirable?
Is HE agar a defined or an undefined medium? It is undefined due to the yeast extract and peptic digest of animal tissue in the medium. An undefined medium is desirable to support growth of a wide variety of the organisms being selected for.
