What are LNCaP cells?

What are LNCaP cells?

LNCaP is a cell line derived from a metastatic lymph node lesion of human prostate cancer which is androgen receptor (AR) positive, exhibits androgen-sensitive growth, and was originally reported by Horoszewicz et al. to form subcutaneous tumors in intact male athymic nude mice at a frequency of 58% [1,2].

Are LNCaP cells adherent?

LNCaP cells are epithelial cells with adherent (and semi-adherent) qualities when growing in cell culture. The LNCaP cell line is sensitive to the hormones estrogen and androgen, which can be used to modulate growth. LNCaP cells are androgen dependent and resistant to human fibroblast interferon when grown in culture.

How do you culture a LNCaP cell?

LNCaP Cell Culture Protocol The base culture medium for LNCaP is RPMI-1640 with 10% FBS. Cells should be maintained in an incubator at 37°C with 5% CO2. To subculture: Rinse cells with 0.25% Trypsin/0.53mM EDTA.

How do cells attach to protein coated substrates?

Cells normally attach to substrates via surface receptors on the cells, which interact with proteins adsorbed onto the surface of the substrate (Hubbell, 1995b). These proteins are adsorbed from either the surrounding serum (culture medium or biological fluid), or secreted by the cells themselves (Saltzman, 1997).

What is the difference between HEK293 and HEK293t cells?

The key difference between HEK293 and HEK293t is that HEK293 is an immortalized cell line derived from the embryonic human kidney that is transfected with sheared human adenovirus type 5DNA while HEK293t is a daughter cell line derived from HEK293 original cell line that is transfected with a plasmid vector carrying …

Why are HEK293t cells used for transfection?

Advantages of HEK293 cells Firstly, they are very easy to grow and to maintain, with high reproducibility, which makes them preferable over other less-robust and slow-growing cell lines. Furthermore, they are very efficient at protein production and accessible for transfection.

What is used for cell adhesion?

Cadherins are essential for cell–cell adhesion and cell signalling in multicellular animals and can be separated into two types: classical cadherins and non-classical cadherins.

How do you increase cell adhesion?

Various groups have reported the enhancement of cell adhesion by increasing surface roughness at the nanometer scale using techniques such as sand blasting. It has also been shown that cell adhesion may be improved by chemical grafting of adhesion peptides such as Arg-Gly-Asp to material surfaces.

Is A549 adherent?

Characteristics. A549 cells, as found in the lung tissue of their origin, are squamous and responsible for the diffusion of some substances, such as water and electrolytes, across alveoli. If A549 cells are cultured in vitro, they grow as a monolayer; adherent or attaching to the culture flask.

Why are hek293t cells used for transfection?

What are HEK293 cells used in?

HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection. They are also used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy as well as safety testing for a vast array of chemicals.