What is auramine stain used for?

What is auramine stain used for?

Rhodamine auramine stain is used for the detection of mycobacteria directly from clinical specimens. The dye binds with the mycolic acids and fluoresces under ultraviolet light. Acid fast organisms (mycobacteria) will appear yellow or orange under ultraviolet light.

What type of stain is auramine O?

fluorochrome stain
Our Auramine O Stain is a fluorochrome stain used in the microscopic detection and examination of acid-fast mycobacteria. Acid-fast organisms have cell walls that are resistant to conventional staining by aniline dyes such as the Gram stain.

What is fluorochrome staining in Mycobacterium aka auramine-rhodamine fluorochrome staining?

Auramine-rhodamine fluorochrome staining also known as “Truant method of staining”, is used to visualize acid-fast bacilli (AFB). Ziehl-Neelsen (hot), Kinyoun (cold) are still widely used methods in developing countries. CDC recommends fluorochrome staining for detecting AFB in primary patient specimens.

What is fluorochrome staining?

Fluorochrome staining: The use of any fluorescent dye (e.g., auramine, rhodamine) used to label or stain. Must be viewed using a fluorescence microscope.

What fluorescence is emitted by rhodamine?

Novel rhodamine dyes excitable with 630 nm laser light and emitting at around 660 nm have been developed. The new rhodamines are very photostable and have high fluorescence quantum yields of up to 80 %, long excited state lifetimes of 3.4 ns, and comparatively low intersystem-crossing rates.

Which dye is used in fluorescence microscopy for tuberculosis?

Background. Light-emitting diode fluorescence microscopy (LED-FM) has been shown to be more sensitive than conventional bright field microscopy using Ziehl-Neelsen (ZN) stain in detecting sputum smear positive tuberculosis in controlled laboratory conditions.

Is auramine fluorescent dye?

Auramine and Rhodamine are both fluorescent dyes with a high affinity for mycolic acid found on the Mycobacterium spp cell wall Hence it stains the cell wall bright yellow or orange, under a fluorescent microscope with a green background. It can also stain parasites with Sporozoa.

What is Fite Faraco stain?

Fite-Faraco stain is the routinely used method to demonstrate Mycobacterium leprae in tissue sections [3]. Though FF staining is more sensitive than ZN method in detection of Mycobacterium leprae in tissue sections, it is not free from flaws [8,9].

What dye is xanthene?

Xanthenes dyes are grouped as diphenylmethane, triphenylmethane, aminohydroxy and fluorescent derivatives. Many uses for these dyes have been reported. They are used as dyes for paper, paints, textiles, anticancer agents, inks, antibacterials, insecticides, biomarkers, and stains.

What does Ziehl Neelsen stain?

Ziehl-Neelsen stain is a staining method to highlight Acid-Alcoholic Resistant Bacilli (AARB). This group of bacilli includes: Mycobacterium tuberculosis, Mycobacterium leprae and other mycobacteria.

When is AFB staining performed?

Your doctor may use an acid-fast bacteria (AFB) stain test to see if you have a lung disease like tuberculosis (TB). In addition to showing tuberculosis, an AFB stain can also show if you have another kind of mycobacterial infection like leprosy or a TB-like disease that can affect people with HIV/AIDS.

What are the stains used for detection of Mycobacterium tuberculosis?

Sputum smear microscopy using the ubiquitous Ziehl-Neelsen (ZN) stain, the primary diagnostic strategy for active tuberculosis (TB) worldwide that is recommended by the World Health Organization (WHO),1 is constrained by its reliance on human skill and time-intensive nature.

What does xanthene mean?

Definition of xanthene 1 : a white crystalline heterocyclic compound C13H10O also : an isomer of this that is the parent of the colored forms of the xanthene dyes. 2 : any of various derivatives of xanthene.

What is xanthene made of?

Xanthene (9H-xanthene, 10H-9-oxaanthracene) is the organic compound with the formula CH2[C6H4]2O. It is a yellow solid that is soluble in common organic solvents. Xanthene itself is an obscure compound, but many of its derivatives are useful dyes.