What is Keio collection?
Table of Contents
What is Keio collection?
The Keio collection (Baba et al, 2006) has been established as a set of single-gene deletion mutants of Escherichia coli K-12. These mutants have a precisely designed deletion from the second codon from the seventh to the last codon of each predicted ORF.
What is gene disruption?
Gene disruption is the simplest form of genome editing that can be achieved using programmable nucleases. DSBs generated by programmable nucleases are predominantly repaired through error-prone NHEJ, which often leads to small insertions and deletions (indels) at or near the cleavage site.
How is gene silencing done?
The genes can be silenced by siRNA molecules that cause the endonucleatic cleavage of the target mRNA molecules or by miRNA molecules that suppress translation of the mRNA molecule. With the cleavage or translational repression of the mRNA molecules, the genes that form them are rendered essentially inactive.
What are attB and attP sites?
The attP site consists of a 234-bp DNA fragment containing five scattered putative Int-binding sites (4) whereas the attB site requires only a 16-bp sequence to be functional. The mv4 minimal attB site is the shortest attB sequence described to date.
Why is lambda DNA used as a standard?
Lambda DNA (48,502 bp) may be used as a molecular weight size marker during nucleic acid gel analysis following digestion with a restriction enzyme (such as HindIII). Lambda DNA can also be used as a substrate in restriction enzyme activity assays.
Is lambda DNA circular or linear?
linear
Our lambda DNA is linear. We do not sell Lambda DNA in a circular form. Theoretically, it could be ligated to form circles but, due to its large size, it is difficult to achieve.
What does K12 strain mean?
E. coli K12, the prokaryotic wimp. Escherichia coli is a gram-negative bacterium that is a normal component of the human intestinal flora, and is ordinarily not a dangerous organism.
What is the difference between knockout and silencing?
Gene silencing is considered a gene knockdown mechanism, which generally reduces the expression of a gene but does not completely eliminate it. In contrast, gene knockout completely erases genes from the organism’s genome; thus, there’s no expression of those genes at all.
