How much does a HiSeq cost?
Table of Contents
How much does a HiSeq cost?
Table 1 Technical specifications of Next Generation Sequencing platforms utilised in this study
| Platform | Illumina MiSeq | Illumina HiSeq 2000 |
|---|---|---|
| Instrument Cost* | $128 K | $654 K |
| Sequence yield per run | 1.5-2Gb | 600Gb |
| Sequencing cost per Gb* | $502 | $41 |
| Run Time | 27 hours*** | 11 days |
How does Illumina HiSeq 2500 work?
The HiSeq 2500 System harnesses proven Illumina SBStechnology to deliver highly accurate data and robust performance for a broad range of applications. SBS uses a reversible-terminator method, with fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands.
How many lanes does the HiSeq 2500 have?
YCGA Sequencer Specifications
| Sequencer | Read length | Bases per lane (Gbp) |
|---|---|---|
| HiSeq 2500 High-output | 2×75 | 30 |
| HiSeq 4000 | 2×100 | 60 |
| HiSeq 4000 | 2×150 | 90 |
| NovaSeq S2 | 2×100 | 330 |
How much does a NovaSeq run cost?
Illumina Services
| Service | Unit | Pricing |
|---|---|---|
| NovaSeq 6000 S4 150 bp PE lane *** | per lane (2.5 Billion reads 2,3) | $4,500.00 |
| NovaSeq 6000 SP 100 bp PE flow cell | per cell (800M reads 2) – 2 independent lanes | $4,750.00 |
| NovaSeq 6000 SP 150 bp PE flow cell | per cell (800M reads 2) – 2 independent lanes | $5,100.00 |
What is a flow cell in NGS?
Flow cell is a channel for adsorbing mobile DNA fragments, and it’s also a core sequencing reactor vessel — all the sequencing happens here. The DNA fragments in the sequencing library will randomly attach to the lanes on the surface of the flow cell when they pass through it.
What is a flow cell?
Flow cells are sample cells designed so that liquid samples can be continuously flowed through the beam path. This is useful for samples that can be damaged by the light source. New sample is continually replenished so that the damage does not interfere with the signal.
