What are THP-1 cells used for?
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What are THP-1 cells used for?
THP-1 is a human leukemia monocytic cell line, which has been extensively used to study monocyte/macrophage functions, mechanisms, signaling pathways, and nutrient and drug transport. This cell line has become a common model to estimate modulation of monocyte and macrophage activities.
How do you revive THP-1 cells?
To revive THP-1 and Jurkat cells from frozen liquid N2 stocks, warm RPMI-1640 supplemented with FBS (10%, complete media) to 37 °C in a water bath. 2. Thaw the capped frozen vial containing cells in a water bath at 37 °C. Note: Only a partial thaw is sufficient, and a small lump of ice should remain in the vial.
Do THP-1 cells adhere?
THP-1 cells are non-adherent, so all treatments you perform to promote their attachment are going to have an impact on them.
Why do THP-1 cells clump?
Popular Answers (1) THP-1 cells grow in clumps as when they divide the new cell attaches to the one it split from. With my THP-1 gentle pipetting up and down will break the clumps but the cells remain viable.
Are THP-1 cells macrophages?
THP-1 cells can be differentiated into macrophage-like cells that resemble properties of mature macrophages by activation of protein kinase C (PKC) with phorbol-12-myristate-13-acetate (PMA), ultimately resulting in cells with increased adherence and loss of proliferative activity (Schwende et al., 1996).
How do you freeze THP-1 cells?
To Successfully Freeze THP-1 Cells, Follow These Tips:
- Thaw frozen vials in a 37°C water bath, but DO NOT LET THE WATER COME IN CONTACT WITH THE LID.
- Transfer the cells to a flask containing warmed media, then transfer some of this back into the vial to get as many cells as you can.
Are THP-1 cells macrophage?
What is a HEK cell?
Human embryonic kidney 293 (HEK293, HEK-293, or HEK) cells are one of the most common cell lines used for research purposes, second only to HeLa cells. Their popularity is due to several properties such as easy maintenance, robustness, and ease of transfection.
How do you prevent cells from clumping?
Minor clumping can be resolved by trituration, or gentle up and down pipetting of cells. Discard contaminated cells and disinfect culture hood and cell culture incubator. Follow good laboratory practices to avoid contamination.
Are THP-1 cells phagocytic?
Results. To identify genes regulating phagocytosis of the bacterium S. aureus, we selected the human monocytic cell line THP-119 as our primary screening model. THP-1 cells have been shown to spontaneously phagocytose bacteria without the need for activation or differentiation20.
What does PMA do to macrophages?
PMA at moderate concentrations induced apoptosis in macrophages, and this process appeared to be increased in the presence of myelin. In contrast, microglia were activated by PMA, and greatly increased their phagocytosis of myelin.
Why are HEK293 cells used for transfection?
Advantages of HEK293 cells Firstly, they are very easy to grow and to maintain, with high reproducibility, which makes them preferable over other less-robust and slow-growing cell lines. Furthermore, they are very efficient at protein production and accessible for transfection.
How do you increase viability in flow cytometry?
Improving Flow Cytometry Obtain meaningful data from viable cells by staining dead ones. Reduce interference from your fluorescent signal. Maintain the fluorescent signal. Use the correct methods for sample preparation.
What is anti clumping agent?
Anti-Clumping Agent is a simple, gentle, concentrated solution which, when added to culture media (usually titrated between a 1:100 and 1:1000 dilution), can reduce cell clumping significantly. Reduced Clumping, Increased Viability, Improved Protein Expression.
Are U937 cells adherent?
Culture of U937 Cells U937 cells were differentiated by culture at a starting cell concentration of 1 × 106 cells/ml in medium containing 100 nM phorbol 12-myristate 13-acetate (PMA) for 48 hr. The cells were then adherent and somewhat flattened.
