What is IC50 in cancer?

What is IC50 in cancer?

Half maximal inhibitory concentration, or IC50, is a measurement representing the halfway point in which a compound of interest produces complete inhibition of a biological or biochemical function.

Which compound is used as an anti-cancer drug?

Cisplatin, or cis-diamminedichloroplatinum(II)[2] (CDDP) is a chemotherapy drug. It was the first member of a class of platinum-containing anti-cancer drugs, which now also includes carboplatin and oxaliplatin.

How is IC50 value calculated for anticancer activity?

The simplest estimate of IC50 is to plot x-y and fit the data with a straight line (linear regression). IC50 value is then estimated using the fitted line, i.e., Y = a * X + b, IC50 = (0.5 – b)/a.

What is IC50 value in cytotoxicity?

The IC50 is defined as “the concentration of an inhibitor where the response (or binding) is reduced by half.” So, if you are testing viability via an MTT assay, the dose of cytotoxic compound at which you achieve 50% viability will be the IC50.

What is IC50 in pharmacology?

Half-maximal inhibitory concentration (IC50) is the most widely used and informative measure of a drug’s efficacy. It indicates how much drug is needed to inhibit a biological process by half, thus providing a measure of potency of an antagonist drug in pharmacological research.

What are IC50 values?

The IC50 value is that concentration of a drug that reduces the activity (or binding) of another drug to an enzyme by 50%. Under certain conditions it can used to express the affinity of the enzyme inhibitor. Drug Interactions. drug-receptor interaction.

What are the properties of anticancer drugs?

Characteristics of Anti-Cancer Drug ADME – Overview

• Absorptive surface area.
• Transit time through the gastrointestinal tract.
• Blood flow to the site of absorption.
• Gastric and intestinal pH and subsequent pH dependent solubility.

What is IC50 of a drug?

Abstract. Half-maximal inhibitory concentration (IC50) is the most widely used and informative measure of a drug’s efficacy. It indicates how much drug is needed to inhibit a biological process by half, thus providing a measure of potency of an antagonist drug in pharmacological research.

What is IC50 Antibody?

Given a titration curve, potency of an antibody is typically quantified as the inhibitory concentration (IC), defined as the antibody concentration at which the viral replication has been reduced by 50% (IC50) or 80% (IC80) relative to the absence of the antibody.

What is a good IC50 value for a drug?

Typically, single digit or sub micromolar IC50 is what most people will look for unless the literature has no other compounds used for the cancer cells. Good luck! Hi, Generally, less than 10 micromolar concentration of a drug in plasma is accepted because higher than 10 uM of a drug inhibit necessary enzymes.

What is a good IC50 for a drug?

Generally, less than 10 micromolar concentration of a drug in plasma is accepted because higher than 10 uM of a drug inhibit necessary enzymes. The most of the drugs on the market have less than 10 micromolar therapeutic plasma concentrations.

What chemical is used in chemotherapy?

Corticosteroids. Corticosteroids, often simply called steroids, are natural hormones and hormone-like drugs that are useful in the treatment of many types of cancer, as well as other illnesses. When these drugs are used as part of cancer treatment, they are considered chemotherapy drugs.

What does high IC50 value mean?

The IC50 value is correlated with drug potency, i.e. the amount of drug necessary to produce the effect—the lower the IC50 value the more potent the drug [33].

Is higher IC50 stronger?

IC50 can be determined with functional assays or with competition binding assays. Sometimes, IC50 values are converted to the pIC50 scale. Due to the minus sign, higher values of pIC50 indicate exponentially more potent inhibitors.

Is higher or lower IC50 better?

The value of f∞ describes drug efficacy—the lower the f∞ the higher the beneficial effect (often denoted by Emax, maximal effect). The IC50 value is correlated with drug potency, i.e. the amount of drug necessary to produce the effect—the lower the IC50 value the more potent the drug [33].