How are flow cytometry results reported?

How are flow cytometry results reported?

The official flow cytometry laboratory report is most commonly an individual-lab-generated, paper report form. A discussion of the potential benefits that might result from the development of improved computerized reporting software and from the increased use of antibody-defined, lineage gating is offered.

What do my flow cytometry results mean?

What Do the Results Mean? Flow cytometry can provide information that is used to diagnose, stage and monitor blood cancers. It can also be used to test for minimal residual disease (MRD), the number of cancer cells remaining in the body after treatment.

How would you describe flow cytometry data?

Flow cytometry data is typically represented in one of two ways: histograms, which measure or compare only a single parameter, and dot-plots which compare 2 or 3 parameters simultaneously on a two- or three-dimensional scatter-plot.

What does flow cytometry data look like?

FACS data are commonly presented as one- dimensional histograms or two-dimensional displays (dot displays or contour maps) with logarithmic axes that extend over a ‘four- to five-decade’ range, representing cells with flourescence values that differ 10,000- to 100,000-fold between the lower and upper ends of the scale.

What is negative flow cytometry?

Negative values mean that the equipment s not well calibrated perhaps control antibodies are not appropriate. You may eventually move the axis to the left. The answer is given by assuming that your cells have the marker you are looking for.

What is a normal flow cytometry?

Flow cytometry is a laser-based technique used to detect and analyze the chemical and physical characteristics of cells or particles. It is most commonly used to evaluate bone marrow, peripheral blood and other fluids in your body.

What is considered positive in flow cytometry?

We can set a marker on the control sample and everything that appears to the right in the test sample is deemed positive. Figure 2. Negative sample (A) and test sample (B).

What does a flow cytometry graph mean?

Flow cytometry tells you the percentage of cells in a particular population that have the characteristics that you are interested in. These characteristics are defined by the array of surface proteins on each cell.

What is normal flow cytometry?

What does negative MFI mean?

What does dim mean in flow cytometry?

The recommended descriptions of antibody fluorescence intensity are “dim” for a uniformly positive population with lower mean fluorescence intensity than a positive normal cell population, “bright” for a uniformly positive population with higher intensity than the control, and “heterogeneous” for antigens that show …

What are the parameters of flow cytometry?

Some other examples of the parameters that can be assessed using flow cytometry are listed below: Measurement of cell pigments such as chlorophyll or phycoerythrin. Measurement of the DNA copy number variation using Flow-FISH (Fluorescent in-situ hybridization) or BACs-on-Beads technology.

What is measured in flow cytometry?

Flow cytometry (FC) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument.

What does MFI measure?

Description. The Money Flow Index (MFI) is a momentum indicator that measures the flow of money into and out of a security over a specified period of time. It is related to the Relative Strength Index (RSI) but incorporates volume, whereas the RSI only considers price.