How are microsatellites used in DNA fingerprinting?
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How are microsatellites used in DNA fingerprinting?
Microsatellites or STRs are repetitive co-dominant sequences of 2–6 bp of DNA that are present throughout the entire genome. They are often used for identification or fingerprinting of DNA. Microsatellites are amplified by PCR using fluorescently labeled primers and the amplicons are separated using CE.
How do microsatellites work?
Microsatellites are simple sequence tandem repeats (SSTRs). The repeat units are generally di-, tri- tetra- or pentanucleotides. For example, a common repeat motif in birds is ACn, where the two nucleotides A and C are repeated in bead-like fashion a variable number of times (n could range from 8 to 50).
What is an SSR marker?
Simple-sequence repeats (SSRs), also known as microsatellites, are short tandem repeated motifs that may vary in the number of repeats at a given locus (Tautz, 1989). SSR markers have many advantages over other molecular markers, such as genetic co-dominance.
What are SSR markers used for?
SSRs have been the most widely used markers for genotyping plants over the past 20 years because they are highly informative, codominant, multi-allele genetic markers that are experimentally reproducible and transferable among related species (Mason, 2015).
Why we use SSR markers?
How do SSR markers work?
SSR genotyping involves the use of simple sequence repeats (SSRs) as DNA markers. SSRs, also called microsatellites, are a type of repetitive DNA sequence ubiquitous in most plant genomes. SSRs contain repeats of a motif sequence 1–6 bp in length.
What are Minisatellites and microsatellites?
Conclusion. Microsatellite is a type of repetitive DNA in the eukaryotic genome with a 2-6 base pairs long repetitive sequences. They are widespread throughout the genome and can be used for paternity testing. On the other hand, minisatellite is a type of repetitive DNA with 10-100 base pairs long repetitive sequences.
