What is an Eppendorf pipette?

What is an Eppendorf pipette?

Eppendorf pipettes are a common laboratory tool used to measure and transport liquid. Due to the cost of these useful instruments, you’ll have to take care to preserve their condition. Pipettes also have different colored tips which represent different dosages.

Are Eppendorf pipettes autoclavable?

The Eppendorf pipettes Research plus and Reference 2 can be fully autoclaved. Following the autoclave cycle, the pipette needs to dry completely and cool down.

What are pipette tips?

Pipette tips are used with pipettes and pipettors to speed processing and reduce cross-contamination. They are available in a variety of materials and styles. Popular pipette styles used are: Universal pipette tips, Filter pipette tips and Low retention pipette tips.

How long should a pipette be put in the UV sterilizer?

Rinse well with distilled water and allow to dry. Treat the pipette parts with Thermo Scientific DNA AWAY Surface Decontaminates according to instructions. Exposure to ultraviolet for 30–60 minutes will further reduce but not completeley eliminate DNA contamination on the pipette surface (4).

How do you Sterilise a pipette?

Autoclaving is the simplest sterilization method if all pipette parts tolerate extreme heat. Pipettes should be autoclaved according to the manufacturer’s instructions. To achieve sterility, a holding time of at least 20 minutes at 121°C (252°F) is required.

How many types of pipettes are there?

Within pipette calibration there are five widely used grades of pipettes, all of which have specific guidelines and requirements regarding use, testing, maintenance, and measurement. The five grades of pipettes include disposable/transfer, graduated/serological, single-channel, multichannel, and repeat pipette.

How do you use Eppendorf Research pipette?

Only immerse the pipette tip in the liquid. > Do not put the pipette down when the pipette tip is filled. > The pipette itself may not come into contact with the liquid. The Eppendorf Research plus pipette is a piston-stroke pipette for aspirating and dispensing liquids.

How do you use a pipette correctly?

Put the pipette—held at 10–45 degrees—against the wall of the receiving chamber, and smoothly depress the plunger to the first stop. Wait one second and then depress the plunger to the second stop. Slide the tip up the vessel wall to remove the pipette. Allow the plunger to return to its rest position.

How do you autoclave Eppendorf pipettes?

Autoclave the pipette at 121°C and 1 bar overpressure for 20 minutes. Afterwards, cool the pipette to ambient temperature and let it dry.

Can UV sterilize plastic?

This depends on the material, wall thickness, planned exposition time and UV wavelength used for disinfection. Most plastic material is not UV-transparent, in this cases the inner side will not be reached by UV radiation. Stay clear of plastics such as polystyrene with aromatic side groups that absorb strongly in U.V.

Why are pipettes so expensive?

These pipettes are expensive because the barrel and the tip are a unit and both are replaced when pipetting.