What is MALDI-TOF microbiology?

What is MALDI-TOF microbiology?

Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) is a powerful analytical mass spectrometry technique that has generated numerous diagnostic and clinical applications, especially for the identification of microorganisms for medical diagnosis.

Is Vitek 2 MALDI-TOF?

Our results showed that MALDI-TOF MS offered higher identification accuracy and lower error rates at the species level when compared to VITEK 2. Additionally, MALDI-TOF MS dramatically shortened identification time from 6-8 hours to just a few minutes.

What is MALDI-TOF analysis?

During MALDI-TOF analysis, the m/z ratio of an ion is measured by determining the time required for it to travel the length of the flight tube. A few TOF analyzers incorporate an ion mirror at the rear end of the flight tube, which serves to reflect back ions through the flight tube to a detector.

How does MALDI-TOF work simple?

In the case of MALDI-TOF, the analyzer separates molecules based on the time it takes each of them to fly through the time-of-flight tube or “drift” region to the detector. It is this travel through the drift region that separates the molecules.

How does Vitek 2 identify bacteria?

PRINCIPLE: The Vitek 2 Compact (30 card capacity) system uses a fluorogenic methodology for organism identification and a turbidimetric method for susceptibility testing using a 64 well card that is barcoded with information on card type, expiration date, lot number and unique card identification number.

How are samples prepared for MALDI?

Mix 1 part saturated HCCA solution with 1 part sample solution. 2. Deposit 0.5 ตL of the matrix/analyte mixture onto the MALDI target and allow to dry. The concentration of the peptide/protein solution should be between 10 fmol – 1 pmol/µL.

How does a Vitek work?

The Vitek 2 Compact (30 card capacity) system uses a fluorogenic methodology for organism identification and a turbidimetric method for susceptibility testing using a 64 well card that is barcoded with information on card type, expiration date, lot number and unique card identification number.

Does Vitek use PCR?

casseliflavus and 21 E. gallinarum. The multiplex PCR, Bruker MS and VITEK MS were able to identify all the isolates correctly at the species level. The VITEK 2 system could identify 131/132 (99.2 %) and 121/132 (91.7 %) of the isolates at the genus and species levels, respectively.

What is the principle of Vitek 2?

How accurate is MALDI-TOF?

The MALDI-TOF MS correctly identified 92% of the M. tuberculosis isolates (95% CI of 0.87 to 0.96), and 68% of M. bovisisolates (95% CI of 27% to 100%) to the species level. Mycobacterium tuberculosis complex in solid media with reference strains using augmented database showing more accurate identification.

What types of sample are suitable for MALDI-TOF mass analysis?

Samples for MALDI analysis may be supplied either in solution or lyophilised. Ideally samples should be resuspended in an aqueous solution of 50% (v/v) acetonitrile, 0.1% (v/v) trifluoroacetic acid using ultra high purity water. If any other solution is used, BioCentre staff must be supplied with comprehensive details.

How does MALDI-TOF MS work?

What is AES in microbiology?

The Advanced Expert System (AES) was used in conjunction with the VITEK 2 automated antimicrobial susceptibility test system to ascertain the beta-lactam phenotypes of 196 isolates of the family Enterobacteriaceae and the species Pseudomonas aeruginosa.