What is the use of Rhodamine B?
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What is the use of Rhodamine B?
As one of the most commonly used dyes, Rhodamine B (RhB) is widely used in industrial purposes, such as printing and dyeing in textile, paper, paints, leathers etc. However, the organic dyes will cause serious environmental and biological problems, even capable to induce irritation to the skin, eyes.
Is Rhodamine b toxic?
The highest lethality observed was 20% at 50 mg/L and 200 mg/L exposure concentrations. Consequently, Rhodamine B was found to be approximately 15 times more toxic than Rhodamine WT based on estimated LC10 values.
Is Rhodamine b water soluble?
Rhodamine B base is also used as a laser dye, tunable around 610 nm with the fluorescence yield being temperature dependent. Solubility : Soluble in ethanol (1 mg/ml), methanol, chloroform, and DMF. Insoluble in water.
Is Rhodamine ba xanthene dye?
Rhodamine B is bright-red in color. Rhodamine B is a xanthene dye, which functions as a water tracer fluorescent. It is used as a staining fluorescent dye.
How do you detect Rhodamine B?
Rhodamine B /ˈroʊdəmiːn/ is a chemical compound and a dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with fluorometers.
What wavelength is rhodamine?
2.9 Lissamine Rhodamine B Sulfonyl Hydrazine The resulting fluorescent derivative exhibits an excitation maximum at a wavelength of 556 nm and a maximal emission wavelength of 580 nm when dissolved in methanol.
Is rhodamine harmful to humans?
A textile dye, Rhodamine B is toxic to the human body. It often enters the body when mixed with food, thereby causing oxidative stress on cells and tissues.
How do you test for Rhodamine B?
Accurate analysis techniques are necessary in order to avoid potential health risks. Merck’s Purospher® HPLC columns are ideal for the detection of Rhodamine B in chili extract. The columns offer highly reliable results, with excellent separations and outstanding peak symmetry.
What type of dye is Rhodamine B?
fluorescent xanthene dye
Another pollutant that is extensively used for dyeing purpose is Rhodamine B (RhB). It is a water-soluble fluorescent xanthene dye used to dye various substances.
Is Rhodamine B an acid or base?
Rhodamine B is a xanthene dye whose optical properties depend of several factors, such as solvent, concentration and pH value [2]. The carboxyl group participates of typical acid-base balance, wherein the acidic and basic forms are strongly luminescent and colored [2].
What wavelength does Rhodamine B absorb?
Rhodamine B is a fluorescent compound with an excitation peak at 546 nm and an emission peak at 568 nm. It can be excited using a 561 nm laser paired with a 586/15 nm bandpass filter, a configuration that can be found, for example, in the BD FACSCelesta™.
What is the difference between rhodamine B and rhodamine 6G?
Rh. B offers a high photostable and efficient laser dye in sol–gel silica sensitized with Rh. 6G; 75,000 shots as a laser half-lifetime of the sample and 24% efficiency at pumping intensity 0.1 J/cm2 of 532 nm. Wavelength shift occurs from 606 to 630 nm in the Rh.
How do you store Rhodamine B?
Storage: Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances. OSHA Vacated PELs: Rhodamine B: No OSHA Vacated PELs are listed for this chemical.
What is the charge of Rhodamine B?
It contains a rhodamine B(1+). C.i. food red 15 appears as green crystals or reddish-violet powder. Used as a dye, especially for paper, as a metal chelating reagent, and in drugs and cosmetics.
How do you make Rhodamine B?
Stock Standard 1 Dissolve 0.01 g of rhodamine B standard in 10 mL of DI water. The concentration of Stock Standard 1 will be 1000 μg/mL. Stock Standard 2 Dilute 200 μL of Stock Standard 1 to 10 mL with DI water. The concentration of Stock Standard 2 will be 20 μg/mL.
Which reagent is used to detect Rhodamine B in chili powder?
Merck’s Purospher® HPLC columns are ideal for the detection of Rhodamine B in chili extract.
How do you dissolve Rhodamine B?
Stock Standard 1 Dissolve 0.01 g of rhodamine B standard in 10 mL of DI water. The concentration of Stock Standard 1 will be 1000 μg/mL. Stock Standard 2 Dilute 200 μL of Stock Standard 1 to 10 mL with DI water.
What color is rhodamine?
The hexadecimal color code #e60094 is a shade of magenta-pink. In the RGB color model #e60094 is comprised of 90.2% red, 0% green and 58.04% blue.
What is rhodamine 6G used for?
Rhodamine 6G /ˈroʊdəmiːn/ is a highly fluorescent rhodamine family dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers.
