Can HCT116 grow in DMEM?
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Can HCT116 grow in DMEM?
Importantly, both HCT116 and NCM460 cell lines were grown in DMEM medium with 10% FBS in all subsequent assays.
Are HCT116 cells adherent?
HCT116 (ATCC® CCL-247™) is a human colorectal carcinoma cell line initiated from an adult male. The cells are adherent with an epithelial morphology. Following implantation into immunocompromised mice, the cells form primary tumors and distant metastases.
Why do we use A549 cells?
The A549 cells have been used to model the alveolar Type II pulmonary epithelium. Studies have shown that this can be particularly useful in research for studying the metabolic processing of lung tissue and for identifying mechanisms of drug delivery to the tissue.
How big are U2OS cells?
We determined the nuclear dimensions of freely growing U2OS cells to be 16 ± 2 μm in width, 23 ± 3 μm in length, and 4 ± 1 μm in height (n = 60).
Are U2OS cells adherent?
All Answers (5) Suspended U2OS cells remain viable and they will re-adhere and keep growing but their growth is likely to be affected while they remain in suspension. The cell cycle length of U2OS cells is ~18–20 h, and so a 48-h time course would be expected to have only ~2.5 cell cycles.
Does Jurkat have TCR?
Jurkat is a CD4+/CD8- T-cell lymphoma that expresses an endogenous TCR.
How long does it take for U2OS cells to adhere?
If you trypsinized the adherent cells then replate them probably only need a few minutes for some of them to attach. For both endothelial and sommoth muscle cells I have seen them attached withing 20 minutes whereas from liquid N2 it may take 2-4 hours.
How do you stimulate a Jurkat cell?
Jurkat cels can be activated by exposure to a combination of anti-CD3/anti-CD28 or phytoheamaggutinin (PHA), or the C305 mAb originally developped by Arthur Weiss. IL-2 production should be measured 6 to 12 h later (whatever works best for you). 0.5 to 2 millions cells per ml works.
