How does Gaussia luciferase work?
Table of Contents
How does Gaussia luciferase work?
Gaussia luciferase is a 20kDa protein from the marine copepod, Gaussia princeps. The bioluminescent enzyme is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity.
Is luciferase dimeric?
Firefly luciferase is a dimer comprising two identical 62 kDa subunits (one active site per dimer, pH optimum 7.75).
What products use luciferase?
Luciferases are widely used in biotechnology, for bioluminescence imaging microscopy and as reporter genes, for many of the same applications as fluorescent proteins.
How is luciferase signal measured?
To measure luciferase activity of the cell lysates, you will need a multiwell plate or a tube containing cell lysates and a luminometer. This apparatus detects the photon emission produced from the luciferase reaction and the unit of measurement comes out as relative light units (RLU).
Is luciferase a fluorescence?
Summary – Luciferase vs GFP Luciferase and GFP are two proteins that are capable of producing bioluminescence. Luciferase is an enzyme that produces light via oxidising its substrate luciferin, while GFP is a protein that displays bright green fluorescence when exposed to light in the blue to ultraviolet range.
Is luciferase monomeric or dimeric?
(≥ 3 mg/ml) luciferase is predominantly dimeric (luciferase is approximately 50% dimeric at 3 mg/ml). At typical working concentrations (0.01 to 1 ng/ml) luciferase is primarily monomeric. The isoelectric point (pI) has been determined to be 6.7 and the pH optimum is 7.8.
Is luciferase a dimeric protein?
Luciferase contains two -SH groups that influence active site conformation but are not required for activity. Firefly luciferase is extremely hydrophobic; the monomer is apparently active, but the enzyme exists as a dimer in most solutions [6].
Does luciferase emit light?
Luciferase is an enzyme. An enzyme is a chemical (called a catalyst) that interacts with a substrate to affect the rate of a chemical reaction. The interaction of the luciferase with oxidized (oxygen-added) luciferin creates a byproduct, called oxyluciferin. More importantly, the chemical reaction creates light.
What is luciferase signal?
A luciferase assay is used to determine if a protein can activate or repress the expression of a target gene.
Is luciferase fluorescent protein?
Luciferase and GFP are two proteins that are capable of producing bioluminescence. Luciferase is an enzyme that produces light via oxidising its substrate luciferin, while GFP is a protein that displays bright green fluorescence when exposed to light in the blue to ultraviolet range.
Is luciferase a monomeric protein?
Recombinant Lucia Luciferase Protein is a monomeric protein expressed in CHO cells. The mature protein is composed of 192 amino acids and has an approximate molecular weight of 24 kDa.
What are dimeric enzymes?
In dimeric enzymes, there are homomeric forms representing the two homozygotes, but the heterozygote occurs in heteromeric form: when stained after electrophoresis, this results in three bands (instead of two bands as with a monomer). An example of a dimeric enzyme is glucose–phosphate isomerase.
Is luciferin hydrophobic?
Firefly luciferase is extremely hydrophobic; the monomer is apparently active, but the enzyme exists as a dimer in most solutions [6].
Does luciferin absorb UV light?
Crystalline luciferin is fluorescent; it absorbs 327-nm UV radiation and emits visible light at 530 nm. Light emitted from fireflies ranges from green (510 nm) to red (670 nm).
