What are CHO cells and what are they used for?

What are CHO cells and what are they used for?

CHO cells, which are a cell line derived from the ovary of the Chinese hamster, meant for use in biological and medical research and commercially in the production of therapeutic proteins.

Why do we use CHO cells?

The Advantages of CHO Cells Grow well in suspension and as adherent culture, rendering the cells ideal for GMP procedures. Their tolerance to variations in pH, oxygen levels, temperature or pressure make them the ideal cell for large-scale culture. High recombinant protein yields and specific productivity.

Are CHO cells cancerous?

CHO cells can also be kept in suspension cultures; in contrast to cancer cells, they are genetically stable; they can be reproduced with expression vectors that contain the “gene of interest” (GOI); they can be transfected; and they remain stable during the process of selection, amplification, single-cell cloning and …

Why are CHO cells important to the pharmaceutical industry?

CHO cells have manifold advantages for the production of complex pharmaceuticals like antibodies. A big asset of CHO cells is their robust growth and adaptability to different media and culture conditions.

Why are CHO cells used for monoclonal antibodies?

Abstract. Chinese hamster ovary (CHO) cells are the preferred production host for therapeutic monoclonal antibodies (mAb) due to their ability to perform post-translational modifications and their successful approval history.

How do they get CHO cells?

The CHO and CHO-K1 cell lines can be obtained from a number of biological resource centres such as the European Collection of Cell Cultures, which is part of the Health Protection Agency Culture Collections.

Is CHO cell line immortal?

Fact #3 – CHO cells are not immortal Well, while they are tiny yet tough, they are not “immortal”. Much rather, they are what is called “immortalized”.

What are NS0 cells?

NS0 cells are a model cell line derived from the nonsecreting murine myeloma used in biomedical research and commercially in the production of therapeutic proteins. The cell line is a cholesterol-dependent cell line that was generated from a subline of NSI/1 which produced only the light chain but no heavy chain.

What is NS0 cell line?

How many times can you passage CHO cells?

Subculture the FreeStyle™ CHO-S™ Cells a minimum of three times to allow them to recover from thawing before using them in transfection experiments.

How are hybridoma cells produced?

Hybridomas are generated by fusing spleen cells (plasma cells) from immunized hosts with a compatible myeloma cell line (malignant and immortal plasma cell line).

What are HEK 293 cells?

293 [HEK-293] is a cell line exhibiting epithelial morphology that was isolated from the kidney of a human embryo. This cell line can be used in industrial biotechnology and toxicology research. It has applications in efficacy testing and viruscide testing.

What are PER C6 cells?

PER. C6 cells are immortalized primary human embryonic retinal cells engineered to express Ad 5 E1A and E1B. The development of matched Ad vectors with no sequence overlap eliminates the RCA problem when packaged in these cells. However, there are many more reasons for the over 25 licensees of Crucell’s PER.

What are Cho S cells?

CHO-S [suspension] A clonal isolate, derived from Chinese hamster ovary (CHO K1) cells. The CHO-S parental cell line was selected for growth and transfection efficiency. CHO-S cells are adapted to serum-free suspension culture.

What have HEK293 cells been used for?

HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection. They are also used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy as well as safety testing for a vast array of chemicals.

What is myeloma cells in hybridoma?

Myeloma cells are preselected to be both nonantibody secreting and sensitive to HAT medium. The resultant fusion product (hybridomas) are thus antibody-secreting and immortal. Unfused myeloma cells die as they cannot survive in the HAT medium, and unfused B cells die as they are not immortal.

Why myeloma cells are used in hybridoma?

Reason: Hybridomas are used to produce monoclonal antibodies. Assertion : Hybridoma cells are shifted to a medium deficient in nutrient which cannot be synthesized by myeloma cells. Reason : This medium allows selection of hybridoma cells.