What is PCR in plant pathology?

What is PCR in plant pathology?

Abstract. Polymerase chain reaction (PCR) is the most widely used nucleo-based method for the detection of plant viruses. It is a primer-mediated in vitro reaction involving amplification of target nucleic acid sequences.

What exactly is PCR used for and why is it an effective and important technique?

PCR is used in molecular biology to make many copies of (amplify) small sections of DNA? or a gene?. Using PCR it is possible to generate thousands to millions of copies of a particular section of DNA from a very small amount of DNA. PCR is a common tool used in medical and biological research labs.

What is the most common form of plant pathogen?

The most common plant pathogens are fungi, bacteria, mollicutes, parasitic higher plants, parasitic green algae, nematodes, protozoa, viruses, and viroids.

What are the techniques involved in DNA based diagnosis?

Efforts to overcome these problems have led to the development of immunoassay and DNA-based diagnostic methods including fluorescent antibody tests (FAT), enzyme-linked immunosorbent assays (ELISA), radioimmunoassay (RIA), in situ hybridization (ISH), dot blot hybridization DBH) and polymerase chain reaction (PCR) …

What is detection and diagnosis of plant disease?

Common methods for the diagnosis and detection of plant diseases include visual plant disease estimation by human raters, microscopic evaluation of morphology features to identify pathogens, as well as molecular, serological, and microbiological diagnostic techniques (Bock et al.

What is plant disease assessment?

Plant disease assessment, or phytopathometry (Large, 1966), involves the measurement and quantification of plant disease and is therefore of fundamental importance in the study and analysis of plant disease epidemics.

Why is plant disease detected?

Detection of plant disease through some automatic technique is beneficial as it reduces a large work of monitoring in big farms of crops, and at very early stage itself it detects the symptoms of diseases i.e. when they appear on plant leaves.

How is PCR test performed?

A healthcare worker will take a sample from inside the back of your throat and nose using a swab. This may be uncomfortable but it won’t be painful. Children under 13 years old will only need to give a nasal swab sample. We will send your sample to a lab for testing.

What are the 4 types of plant pathogens?

The plant pathogens comprise viruses, bacteria, fungi, nematode, and parasitic plant.

What are the applications of PCR?

We present a survey of the following applications of PCR: 1) The amplification of gene fragments as fast alternative of cloning. 2) The modification of DNA fragments. 3) The sensitive detection of pathogenic microorganisms, if desired followed by an accurate genotyping. 4) DNA analysis of arachaeological specimens.

What are the objectives of plant disease detection?

Thus the main objectives are: 1) To design such system that can detect crop disease and pest accurately. 2) Create database of insecticides for respective pest and disease. 3) To provide remedy for the disease that is detected.

What type of antibodies can be used in testing kits to detect plant diseases?

Samples of the plant tissue can be tested with the monoclonal antibody. If the pathogen’s antigens are present in the plant tissue then the monoclonal antibody sticks to it.

How are plant diseases measured?

The simplest method of disease measurement is to estimate the percentage infection. For wide surveys the records may take the form of the percentage of crops in a district in which a disease occurs, and may be designated as P (disease prevalence).