What is the predicted size for GFP?
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What is the predicted size for GFP?
Protein size and shape matters One of the limitations is the size of GFP: ~240 amino acids or about 28 kDa.
What is the size of GFP protein?
GFP is a 28 kDa protein that resembles a cylinder with a length of 4.2 nm and a diameter of about 2.4 nm (Hink et al., 2000). The complete beta-barrel is necessary for its fluorescence and therefore GFP cannot be downsized by deleting residues.
What is the DNA sequence for GFP?
Proteins that fluoresce contain components called fluorophores. In GFP the fluorophore originates from an internal tripeptide sequence (Ser‐Tyr‐Gly) which is post‐translationally modified to the structure shown below, a 4‐(p‐hydroxybenzylidene)‐imidazolidin‐5‐one.
How big is the GFP gene?
around 730 base-pairs long
Most commercially available genes for GFP and similar fluorescent proteins are around 730 base-pairs long. The natural protein has 238 amino acids. Its molecular mass is 27 kD.
Why is protein size important?
Background. The sizes of proteins are relevant to their biochemical structure and for their biological function. The statistical distribution of protein lengths across a diverse set of taxa can provide hints about the evolution of proteomes.
How much kDa is GFP?
26.9 kD
With GFP, the primary structure is 239 amino acids with a total molecular weight of 26,870 daltons, or 26.9 kD.
How many base pairs is the GFP gene?
730 bp
One interesting candidate gene that fulfills these requirements is the gene-encoding green fluorescent protein (GFP). It was originally isolated from the jellyfish Aquorea victoria. The GFP cDNA consists of 730 bp, which encode a 238 amino acid protein with a molecular weight of 27 kD (2).
What is the size of the native green fluorescent protein in kDa?
How is protein size determined?
Whatever program you use to visualize protein structures should have tool to measure the distance between any 2 atoms in the structure. Find the 2 atoms that appear to be the farthest apart, and measure the distance between them. That is the size of the protein in that dimension.
What is protein size?
A simple rule of thumb for thinking about typical soluble proteins like the Rubisco monomer is that they are 3-6 nm in diameter as illustrated in Figure 1 which shows not only Rubisco, but many other important proteins that make cells work.
How big is mCherry?
26.722 kDa
Structure. The gene for mCherry is 711bp long, and the protein is made up of 236 residues with a mass of 26.722 kDa.
What is the smallest fluorescent tag?
With molecular weight of only 17 kDa, miRFP670nano is the smallest monomeric NIR FP that fluoresces in mammalian cells as bright as twice bigger state-of-art two-domain NIR FPs.
How big is HaloTag?
33 kDa
HaloTag is a self-labeling protein tag. It is a 297 residue protein (33 kDa) derived from a bacterial enzyme, designed to covalently bind to a synthetic ligand.
How do you tag a gene with GFP?
Popular Answers (1)
- Use a vector, place your gene in front of the GFP gene. The stop codon of your gene should be removed, and your gene and the GFP gene should be in frame.
- Attached a paper for you.
- Also refer to another RG post for the similar topic with other persons’ suggestions.
